A MICROBIOLOGICAL ASSAY TECHNIQUE FOR PANTOTHENIC ACID WITH THE USE OF PROTEUS MORGANII
نویسندگان
چکیده
منابع مشابه
A Microbiological Assay Technique for Panto- Thenic Acid with the Use of Proteus Morganii”
Several methods for the assay of pantothenic acid have been reported in the literature. They have been based on the growth response of yeast (l), bacteria (2, 3), and chicks (4, 5) to the vitamin. The relative merits of these methods have been discussed in an earlier paper (3). In a previous publication (6) it was shown that pantothenic acid is one of the growth factors required by Proteus morg...
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Before the nature of “bound” pantothenic acid was established, McIlwain and Hughes (1) showed that incubation of pantothenic acid with various streptococci or Proteus morganii led to its “inactivation” as a growth factor for these organisms. This inactivation reaction was dependent upon glycolysis, but independent of growth and the presence of oxygen, and was inhibited by pantoyltaurine (2). Mc...
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Lipmann, Kaplan, Novelli, Tuttle, and Guirard (1) reported that, whereas concentrates of the coenzyme required for acetylation (coenzyme A) showed no pantothenic acid activity by the ordinary microbiological assay, considerable amounts of p-alanine were found after acid hydrolysis. From this early observation, it was suspected that the coenzyme might contain combined pantothenic acid which was ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1941
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)51368-0